オバタ　ヒデアキ   OBATA Hideaki   小幡　英章    所属   埼玉医科大学  医学部 総合医療センター　麻酔科（麻酔科、産科麻酔科）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Spinal dopaminergic involvement in the antihyperalgesic effect of antidepressants in a rat model of neuropathic pain
掲載誌名	正式名：NEUROSCIENCE LETTERS ISSNコード：03043940
出版社	ELSEVIER IRELAND LTD
巻・号・頁	649,116-123頁
著者・共著者	Mi Chen,Hajime Hoshino,Shigeru Saito,Yang Yang,Hideaki Obata
発行年月	2017/05
概要	Antidepressants such as tricyclic antidepressants, and serotonin noradrenaline reuptake inhibitors are a first-line treatment for neuropathic pain. Here, we aimed to determine the involvement of the spinal dopaminergic system in the antihyperalgesic effects of antidepressants in a rat model of neuropathic pain induced by spinal nerve ligation (SNL). The right L5 spinal nerve of male Sprague-Dawley rats was ligated under inhalation anesthesia to induce hyperalgesia. Behavioral testing was performed by measuring ipsilateral hindpaw withdrawal thresholds after intraperitoneal injection of amitriptyline, duloxetine, milnacipran, and fluoxetine. D2-like receptors were blocked by intrathecal administration of sulpiride. We also determined the concentrations of dopamine in the spinal cord using microdialysis after injection of antidepressants. The dopamine contents in the spinal dorsal horn were also measured in normal and SNL rats at 2, 3, 4, and 8 weeks after SNL surgery. Intraperitoneal injection of amitriptyline, duloxetine, milnacipran, and fluoxetine (3-30 mg/kg) produced antihyperalgesic effects, and prevented by intrathecal pre-injection of sulpiride (30 mu g). Microdialysis revea
DOI	10.1016/j.neulet.2017.04.017
PMID	28416380