オバタ　ヒデアキ   OBATA Hideaki   小幡　英章    所属   埼玉医科大学  医学部 総合医療センター　麻酔科（麻酔科、産科麻酔科）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Relief of Hypersensitivity after Nerve Injury from Systemic Donepezil Involves Spinal Cholinergic and gamma-Aminobutyric Acid Mechanisms
掲載誌名	正式名：ANESTHESIOLOGY ISSNコード：00033022
出版社	LIPPINCOTT WILLIAMS&WILKINS
巻・号・頁	118(1),173-180頁
著者・共著者	Masafumi Kimura,Ken-ichiro Hayashida,James C. Eisenach,Shigeru Saito,Hideaki Obata
発行年月	2013/01
概要	Background: Evoking spinal release of acetylcholine (ACh) produces antinociception in normal animals and reduces hypersensitivity after nerve injury, and some studies suggest that ACh-mediated analgesia relies on gamma-aminobutyric acid (GABA)-ergic signaling in the spinal cord. In this study, the authors tested the spinal mechanisms underlying the anti-hypersensitivity effects of donepezil, a central nervous system-penetrating cholinesterase inhibitor, in a rat model of neuropathic pain.Methods: Male Sprague-Dawley rats were anesthetized, and L5 spinal nerve ligation was performed unilaterally. Withdrawal threshold to a paw pressure test was measured before and after intraperitoneal administration of donepezil, with or without intrathecal antagonists for cholinergic and GABAergic receptors. Microdialysis studies in the ipsilateral dorsal horn of the lumbar spinal cord were also performed to measure extracellular ACh and GABA.Results: Donepezil increased the withdrawal threshold in spinal nerve ligation rats but not in normal rats. The anti-hypersensitivity effect of donepezil (1 mg/kg) in spinal nerve ligation rats was reduced by intrathecal pretreatment with atropine (30 mu g),
DOI	10.1097/ALN.0b013e318277a81c
PMID	23221863