オバタ　ヒデアキ   OBATA Hideaki   小幡　英章    所属   埼玉医科大学  医学部 総合医療センター　麻酔科（麻酔科、産科麻酔科）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Ondansetron Reverses Antihypersensitivity from Clonidine in Rats after Peripheral Nerve Injury Role of gamma-Aminobutyric Acid in alpha 2-Adrenoceptor and 5-HT3 Serotonin Receptor Analgesia
掲載誌名	正式名：ANESTHESIOLOGY ISSNコード：00033022
出版社	LIPPINCOTT WILLIAMS&WILKINS
巻・号・頁	117(2),389-398頁
著者・共著者	Ken-ichiro Hayashida,Masafumi Kimura,Masaru Yoshizumi,Shotaro Hobo,Hideaki Obata,James C. Eisenach
発行年月	2012/08
概要	Introduction: Monoaminergic pathways, impinging an alpha 2-adrenoceptors and 5-HT3 serotonin receptors, modulate nociceptive transmission, but their mechanisms and interactions after neuropathic injury are unknown. Here we examine these interactions in rodents after nerve injury.Methods: Male Sprague-Dawley rats following L5-L6 spinal nerve ligation (SNL) were used for either behavioral testing, in vivo microdialysis for gamma-aminobutyric acid (GABA) and acetylcholine release, or synaptosome preparation for GABA release.Results: Intrathecal administration of the alpha 2-adrenoceptor agonist (clonidine) and 5-HT3 receptor agonist (chlorophenylbiguanide) reduced hypersensitivity in SNL rats via GABA receptor-mediated mechanisms. Clonidine increased GABA and acetylcholine release in vivo in the spinal cord of SNL rats but not in normal rats. Clonidine-induced spinal GABA release in SNL rats was blocked by alpha 2-adrenergic and nicotinic cholinergic antagonists. The 5-HT3 receptor antagonist ondansetron decreased and chlorophenylbiguanide increased spinal GABA release in both normal and SNL rats. In synaptosomes from the spinal dorsal horn of SNL rats, presynaptic GABA release was
DOI	10.1097/ALN.0b013e318260d381
PMID	22722575