オバタ　ヒデアキ   OBATA Hideaki   小幡　英章    所属   埼玉医科大学  医学部 総合医療センター　麻酔科（麻酔科、産科麻酔科）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Milnacipran inhibits glutamatergic N-Methyl-D-Aspartate receptor activity in Spinal Dorsal Horn Neurons
掲載誌名	正式名：MOLECULAR PAIN ISSNコード：17448069
出版社	BIOMED CENTRAL LTD
巻・号・頁	8,45頁
著者・共著者	Tatsuro Kohno,Masafumi Kimura,Mika Sasaki,Hideaki Obata,Fumimasa Amaya,Shigeru Saito
発行年月	2012/06
概要	Background: Antidepressants, which are widely used for treatment of chronic pain, are thought to have antinociceptive effects by blockade of serotonin and noradrenaline reuptake. However, these drugs also interact with various receptors such as excitatory glutamatergic receptors. Thermal hyperalgesia was induced by intrathecal injection of NMDA in rats. Paw withdrawal latency was measured after intrathecal injection of antidepressants. The effects of antidepressants on the NMDA and AMPA-induced responses were examined in lamina II neurons of rat spinal cord slices using the whole-cell patch-clamp technique. The effects of milnacipran followed by application of NMDA on pERK activation were also investigated in the spinal cord.Results: Intrathecal injection of milnacipran (0.1 mu mol), but not citalopram (0.1 mu mol) and desipramine (0.1 mu mol), followed by intrathecal injection of NMDA (1 mu g) suppressed thermal hyperalgesia. Milnacipran (100 mu M) reduced the amplitude of NMDA (56 +/- 3 %, 64 +/- 5 % of control)-, but not AMPA (98 +/- 5 %, 97 +/- 5 % of control)- mediated currents induced by exogenous application and dorsal root stimulation, respectively. Citalopram (100 mu M) a
DOI	10.1186/1744-8069-8-45
PMID	22716121