オバタ　ヒデアキ   OBATA Hideaki   小幡　英章    所属   埼玉医科大学  医学部 総合医療センター　麻酔科（麻酔科、産科麻酔科）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Normal glucose enhances neuronal regeneration after lidocaine-induced injury
掲載誌名	正式名：BRITISH JOURNAL OF ANAESTHESIA ISSNコード：00070912
出版社	OXFORD UNIV PRESS
巻・号・頁	104(4),482-486頁
著者・共著者	A. Abdel Nazeer,S. Saito,S. Sayed,L. Hassan,F. Askar,W. Al-jahdari,T. Seki,Obata H
発行年月	2010/04
概要	Local anaesthetics exhibit direct neurotoxic effects on neurones. Numerous studies have investigated the factors that may reverse this neuropathology, but the effects of glucose conditions on neuronal regeneration after lidocaine-induced injury have not been examined by observing living neurones. The present study investigated the effects of different glucose conditions on neurite length, growth cone regeneration, and cell death in dorsal root ganglia (DRG) neurones after lidocaine-induced injury in vitro.DRG explants were isolated from chick embryos at embryonic day 8 and cultured in media containing low, normal, or high glucose concentrations (10, 25, or 40 mM) for 24 h. Tissues were exposed to lidocaine 8 mM for 1 h, then rinsed and incubated for a further 24 h. Neurite length and growth cone collapse assays were performed to assess neuronal growth and regeneration. Lactate dehydrogenase (LDH) and caspase assays were also performed to detect neuronal cell death.Addition of lidocaine for 1 h resulted in> 97% growth cone collapse and neurite destruction under all three glucose conditions. Two hours after rinsing out the lidocaine, significant reversal of growth cone collapse
DOI	10.1093/bja/aeq030
PMID	20194355