トクモト ヤスヒト
TOKUMOTO Yasuhiro
徳元 康人 所属 埼玉医科大学 医学部 アドミッションセンター 職種 専任講師 |
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論文種別 | 学術雑誌(原著) |
言語種別 | 英語 |
査読の有無 | 査読あり |
表題 | Induction of memory-like CD8+T cells and CD4+T cells from human naive T cells in culture |
掲載誌名 | 正式名:Clinical and experimental immunology 略 称:Clin Exp Immunol ISSNコード:00099104/13652249 |
掲載区分 | 国外 |
出版社 | Oxford University Press |
巻・号・頁 | 207(1),95-103頁 |
総ページ数 | 8 |
著者・共著者 | @Yasuhito Tokumoto, Yasuto Araki, Yusuke Narizuka, Yosuke Mizuno, Susumu Ohshima, Toshihide Mimura |
発行年月 | 2021/11/18 |
概要 | We describe a method to produce human memory-like T cells from naïve human T cells in culture. Using commercially available human T cell differentiation kits, both purified naïve CD8+ T cells and purified naïve CD4+ T cells were activated via T cell receptor signaling and appropriate cytokines for several days in culture. All the T cell activators were then removed from the medium and the cultures were continued in hypoxic condition (1% O2 atmosphere) for several more days; during this period, most of the cells died, but some survived in a quiescent state for a month. The survivors had small round cell bodies, expressed differentiation markers characteristic of memory T cells and restarted proliferation when the T cell activators were added back. |
researchmap用URL | https://doi.org/10.1093/cei/uxab012 |