カイダ　ケンイチ   KAIDA Kenichi   海田　賢一    所属   埼玉医科大学  医学部 総合医療センター　脳神経内科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Harmful effects of anti-GalNAc-GD1a antibodies and TNF-α on rat dorsal root ganglia
掲載誌名	正式名：Journal of the Peripheral Nervous System ISSNコード：10859489
巻・号・頁	10(2),190-201頁
著者・共著者	Keiko Kamakura,Ken-Ichi Kaida,Susumu Kusunoki,Norikazu Miyamoto,Toshihiro Masaki,Ryohji Nakamura,Kazuo Motoyoshi,Jun Fukuda
発行年月	2005/06
概要	The clinical characteristics of five (22%) of 23 patients with Guillain-Barré syndrome (GBS), whose serum contained immunogiobulin G (IgG) antibodies to the ganglioside N-acetylgalactosaminyl GD1a (GalNAc-GD1a), included pure motor weakness of the axonal type. These patients had a relatively good prognosis, but displayed higher serum tumor necrosis factor-alpha (TNF-α) titers than the other GBS patients. We examined the effect of serum from these patients with IgG anti-GalNAc-GD1a antibodies on neurites from cultured rat dorsal root ganglia (DRG) and found it to damage the myelin in well-elongated DRG neurites and monolayer cultures of Schwann cells and neurons. In the regeneration model, serum from these patients delayed neurite extension and inhibited Schwann cell proliferation. Neurons in cultured monolayers showed vacuolation and decreased rapidly in number. Schwann cells were also vacuolated and readily detached from the substratum. The effects of IgG anti-GalNAc-GD1a antibodies purified from one of the patients, rabbit serum after immunization with GalNAc-GD1a, and recombinant TNF-α were also examined. IgG anti-GalNAc-GD1a antibodies mainly inhibited the regeneration and p
DOI	10.1111/j.1085-9489.2005.0010209.x
PMID	15958130