コレマツ　セイゴ   KOREMATSU Seigo   是松　聖悟    所属   埼玉医科大学  医学部 総合医療センター　小児科（小児科､総合周産期母子医療センター新生児科、小児救命救急センター）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Distinct aggregation of beta- and gamma-chains of the high-affinity IgE receptor on cross-linking
掲載誌名	正式名：JOURNAL OF HISTOCHEMISTRY&CYTOCHEMISTRY ISSNコード：00221554
出版社	HISTOCHEMICAL SOC INC
巻・号・頁	48(12),1705-1715頁
著者・共著者	K Asai,K Fujimoto,M Harazaki,T Kusunoki,S Korematsu,C Ide,C Ra,S Hosoi
発行年月	2000/12
概要	The high-affinity IgE receptor (Fc epsilon RI) on mast cells and basophils consists of a ligand-binding alpha -chain and two kinds of signaling chains, a beta -chain and disulfide-linked homodimeric gamma -chains. Crosslinking by multivalent antigen results in the aggregation of the bound IgE/alpha -chain complexes at the cell surface, triggering cell activation, and subsequent internalization through coated pits. However, the precise topographical alterations of the signaling beta- and gamma -chains during stimulation remain unclarified despite their importance in ligand binding/signaling coupling. Here we describe the dynamics of Fc epsilon RI subunit distribution in rat basophilic leukemia cells during stimulation as revealed by immunofluorescence and immunogold electron microscopy. Immunolocalization of beta- and gamma -chains was homogeneously distributed on the cell surfaces before stimulation, while crosslinking with multivalent antigen, which elicited optimal degranulation, caused a distinct aggregation of these signaling chains on the cell membrane. Moreover, only gamma- but not beta -chains were aggregated during the stimulation that evoked suboptimal secretion. These fin