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コレマツ セイゴ
KOREMATSU Seigo
是松 聖悟 所属 埼玉医科大学 医学部 総合医療センター 小児科（小児科､総合周産期母子医療センター新生児科、小児救命救急センター） 職種 教授 |
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| 論文種別 | 学術雑誌(原著) |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Comparison of skin prick test with serially diluted wild-type and genetically engineered recombinant Der f2 |
| 掲載誌名 | 正式名：ANNALS OF ALLERGY ASTHMA&IMMUNOLOGY ISSNコード：10811206 |
| 出版社 | AMER COLL ALLERGY ASTHMA IMMUNOLOGY |
| 巻・号・頁 | 84(3),366-368頁 |
| 著者・共著者 | T Kusunoki,Y Inoue,S Korematsu,M Harazaki,T Yokota,S Hosoi |
| 発行年月 | 2000/03 |
| 概要 | Background: C8/119S, a genetically engineered less allergenic mutant of group II allergen (Der f2) of house dust mite, Dermatophagoides farinae, was constructed in order to reduce the risk of anaphylactic reactions of allergen specific immunotherapy.Objective: To further evaluate, with a larger number of mite-allergic patients, the safety of C8/119S for the treatments of humans.Method: We tested the dose-dependent responses of 20 mite-allergic volunteers to a skin prick test with C8/119S and wild-type recombinant Der f2 and compared the biologic potentials of these allergens to induce type I allergic reactions. In a separate experiment, we compared IgE binding capacities to C8/119S and to wild-type recombinant Der f2 in individual sera from 34 mite-allergic donors.Results: The concentration of C8/119S needed to induce positive skin prick test (SPT) reaction was at least 100 times more than that of recombinant Der f2 in 95% of the volunteers tested. Consistent with this result, IgE binding data showed that 85% of the mite-allergic donors had little or no detectable IgE bound to C8/119S. Our data also shows that a minority of mite-allergic patients responded in a similar manner to |