イチキ　ヨシノブ   ICHIKI Yoshinobu   市来　嘉伸    所属   埼玉医科大学  医学部 国際医療センター　呼吸器外科    職種   専任講師
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読なし
表題	Simultaneous cellular and humoral immune response against mutated p53 in a patient with lung cancer.
掲載誌名	正式名：Journal of immunology (Baltimore, Md. : 1950) ISSNコード：00221767
巻・号・頁	172(8),4844-50頁
著者・共著者	Yoshinobu Ichiki,Mitsuhiro Takenoyama,Makiko Mizukami,Tetsuya So,Masakazu Sugaya,Manabu Yasuda,Tomoko So,Takeshi Hanagiri,Kenji Sugio,Kosei Yasumoto
発行年月	2004/04
概要	We recently identified several Ags recognized by tumor-infiltrating B lymphocyte-derived Ab using SCID mice and a xenografted non-small cell lung cancer system. One of these identified Ags was mutatedp53 with a point mutation resulting in the alteration of codon 158 from Arg to Leu. The aim of this study was to ascertain whether cellular immunity against mutated p53 exists in the same patient together with humoral immunity. Two different nona peptides (mutated p53(150) and p53(155) peptides), including a mutated amino acid derived from p53, were synthesized according to the binding motif of HLA class I of the established cancer cell line A904L from the patient. Mediastinal lymph node lymphocytes of the patient were stimulated weekly with the peptides. The mutated p53(155) peptide-stimulated lymphocytes showed specific cytotoxicity against both autologous EBV-transformed B cells pulsed with mutated p53(155) peptide and A904L. The mutated p53(155) peptide-specific CTL clone in an HLA-Cw*0702 restriction was established and analyzed for its TCR usage. Clonotypic PCR using CDR3-specific primers was applied to the tumor tissue containing the tumor-infiltrating lymphocytes. The specific
PMID	15067062