イチキ　ヨシノブ   ICHIKI Yoshinobu   市来　嘉伸    所属   埼玉医科大学  医学部 国際医療センター　呼吸器外科    職種   専任講師
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読なし
表題	Identification of a lung cancer antigen evading CTL attack due to loss of human leukocyte antigen (HLA) class I expression.
掲載誌名	正式名：Cancer science
巻・号・頁	101(10),2115-20頁
著者・共著者	Tetsuro Baba,Takeshi Hanagiri,Mitsuhiro Takenoyama,Hironobu Shiota,Koji Kuroda,Yoshiki Shigematsu,Yoshinobu Ichiki,Hidetaka Uramoto,Tomoko So,Kosei Yasumoto
発行年月	2010/10
概要	The human lung cancer cell line, C831L, lost HLA class I expression due to a mutation of the β2-microglobulin (β2m) gene, and it may have been the result of immunoediting by CTL cytotoxicity. By restoration of HLA class I expression, we could identify the antigen that may be associated with HLA downregulation. Such an antigen might be a promising target of immunotherapy because it potentially may induce a sufficient immune response to eradicate cancer cells. The CTL clone could be established from lymph node lymphocytes in patient C831 by stimulation with wild-type β2m-transduced C831L (C831L-wβ2m). The CTL clone showed reactivity against C831L-wβ2m in a HLA-B*0702-restricted manner, but not Parental-C831L or autologous normal cells. The cDNA expression cloning method was used to identify the antigen coding gene recognized by the CTL clone. The cDNA clone exhibited a homology with a part of the mRNA that codes for leucine rich repeat containing eight family member A (LRRC8A). A transfection analysis of minigenes indicated that the antigen peptide was derived from protein translated from the downstream of the registered open reading frame in LRRC8A mRNA. The antigenic 9-mer pep
DOI	10.1111/j.1349-7006.2010.01659.x
PMID	20649604