イチキ　ヨシノブ   ICHIKI Yoshinobu   市来　嘉伸    所属   埼玉医科大学  医学部 国際医療センター　呼吸器外科    職種   専任講師
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読なし
表題	Detection of circulating tumor cells with a novel microfluidic system in malignant pleural mesothelioma.
掲載誌名	正式名：Cancer science
巻・号・頁	110(2),726-733頁
著者・共著者	Kazue Yoneda,Taiji Kuwata,Yasuhiro Chikaishi,Masataka Mori,Masatoshi Kanayama,Masaru Takenaka,Soichi Oka,Ayako Hirai,Naoko Imanishi,Koji Kuroda,Yoshinobu Ichiki,Takashi Ohnaga,Fumihiro Tanaka
発行年月	2019/02
概要	Detection of rare tumor cells circulating in the blood (CTCs) presents technical challenges. CellSearch, the only approved system for clinical use, fails to capture epithelial cell adhesion molecule-negative CTCs such as malignant pleural mesothelioma (MPM). We have developed a novel microfluidic device (CTC-chip) in which any Ab to capture CTCs is conjugated. The CTC-chip was coated with an Ab against podoplanin that is abundantly expressed on MPM. Circulating tumor cell-detection performance was evaluated in experimental models in which MPM cells were spikedin blood sampled from a healthy volunteer and in clinical samples drawn from MPM patients. The CTC-chip showed superior CTC-detection performance over CellSearch in experimental models (sensitivity, 63.3%-64.5% vs 0%-1.1%; P < .001) and in clinical samples (CTC-positivity, 68.8% vs 6.3%; P < .001). A receiver operating characteristic (ROC) analysis showed that the CTC test provided a significant diagnostic performance in discrimination of unresectable disease from resectable disease (area under the ROC curve, 0.851; P = .003). The higher CTC count (≥2 cells/mL) was significantly associated with a poor prognosis (P =
DOI	10.1111/cas.13895
PMID	30499156