オノ　コウジ   ONO Koji   小野　公嗣    所属   埼玉医科大学  保健医療学部 臨床検査学科    職種   准教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Functional Roles and Pathophysiology of Brain D-Amino Acid Oxidase
掲載誌名	正式名：Flavins and Flavoproteins 2005
巻・号・頁	853-860頁
著者・共著者	Kiyoshi Fukui,Hwan Ki Park,Tomoya Kawazoe,Koji Ono,Sanae Iwana,Yumiko Tomita,Kazuko YORITA,Takashi Sakai,Young Ho Kim
発行年月	2005/12
概要	D-Amino acid oxidase (D-amino acid: O2 oxidoreductase, DAO; EC 1.4.3.3) is a flavoenzyme with noncovalently bound FAD as its prosthetic group, that catalyzes the oxidative deamination of wide range of D-amino acids to the corresponding imino acids, whereas molecular oxygen undergoes reduction tohydrogen peroxide (H2O2) . The imino acid is nonenzymatically hydrolyzed to α-keto acid and ammonia. H2O2, a type of reactive oxygen species (ROS), is known to damage DNA, proteins and lipids, thereby leading to stress-induced apoptosis. In mammals, DAO is found at highest concentration in kidney proximal tubules, liver and granules of neutrophilic leukocyte as well as certain parts of the brain. This enzyme is intracellularly localized in peroxisomes, and DAO is regarded as a characteristic marker enzyme of the peroxisomes. Since the initial characterization of this enzyme, many attempts have been made to clarify the physicochemical properties and reaction mechanism.