ジュウジョウ　ケンタロウ   JUJO Kentaro   重城　健太郎    所属   埼玉医科大学  医学部 総合医療センター　心臓内科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Improved Culture-Based Isolation of Differentiating Endothelial Progenitor Cells from Mouse Bone Marrow Mononuclear Cells
掲載誌名	正式名：PLOS ONE ISSNコード：19326203
出版社	PUBLIC LIBRARY SCIENCE
巻・号・頁	6(12),e28639頁
著者・共著者	Haruki Sekiguchi,Masaaki Ii,Kentaro Jujo,Ayumi Yokoyama,Nobuhisa Hagiwara,Takayuki Asahara
発行年月	2011/12
概要	Numerous endothelial progenitor cell (EPC)-related investigations have been performed in mouse experiments. However, defined characteristics of mouse cultured EPC have not been examined. We focused on fast versus slow adherent cell population in bone marrow mononuclear cells (BMMNCs) in culture and examined their characteristics. After 24 h-culture of BMMNCs, attached (AT) cells and floating (FL) cells were further cultured in endothelial differentiation medium separately. Immunological and molecular analyses exhibited more endothelial-like and less monocyte/ macrophage-like characteristics in FL cells compared with AT cells. FL cells formed thick/ stable tube and hypoxia or shear stress overload further enhanced these endothelial-like features with increased angiogenic cytokine/ growth factor mRNA expressions. Finally, FL cells exhibited therapeutic potential in a mouse myocardial infarction model showing the specific local recruitment to ischemic border zone and tissue preservation. These findings suggest that slow adherent (FL) but not fast attached (AT) BMMNCs in culture are EPC-rich population in mouse.
DOI	10.1371/journal.pone.0028639