ジュウジョウ　ケンタロウ   JUJO Kentaro   重城　健太郎    所属   埼玉医科大学  医学部 総合医療センター　心臓内科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Enhanced potency of cell-based therapy for ischemic tissue repair using an injectable bioactive epitope presenting nanofiber support matrix
掲載誌名	正式名：JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY ISSNコード：00222828
出版社	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
巻・号・頁	74,231-239頁
著者・共著者	Joern Tongers,Matthew J. Webber,Erin E. Vaughan,Eduard Sleep,Marie-Ange Renault,Jerome G. Roncalli,Ekaterina Klyachko,Tina Thorne,Yang Yu,Katja-Theres Marquardt,Christine E. Kamide,Aiko Ito,Sol Misener,Meredith Millay,Ting Liu,Kentaro Jujo,Gangjian Qin,Douglas W. Losordo,Samuel I. Stupp,Raj Kishore
発行年月	2014/09
概要	The translation of cell-based therapies for ischemic tissue repair remains limited by several factors, including poor cell survival and limited target site retention. Advances in nanotechnology enable the development of specifically designed delivery matrices to address these limitations and thereby improve the efficacy of cell-based therapies. Given the relevance of integrin signaling for cellular homeostasis, we developed an injectable, bioactive peptide-based nanofiber matrix that presents an integrin-binding epitope derived from fibronectin, and evaluated its feasibility as a supportive artificial matrix for bone marrow-derived pro-angiogenic cells (BMPACs) used as a therapy in ischemic tissue repair. Incubation of BMPACs with these peptide nanofibers in vitro significantly attenuated apoptosis while enhancing proliferation and adhesion. Pro-angiogenic function was enhanced, as cells readily formed tubes. These effects were, in part, mediated via p38, and p44/p42 MAP kinases, which are downstream pathways of focal adhesion kinase. In a murine model of hind limb ischemia, an intramuscular injection of BMPACs within this bioactive peptide nanofiber matrix resulted in greater rete
DOI	10.1016/j.yjmcc.2014.05.017