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カンダ ショウイチロウ
KANDA Shoichiro
神田 祥一郎 所属 埼玉医科大学 医学部 小児科（小児科、新生児科） 職種 教授 |
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| 論文種別 | 学術雑誌(原著) |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Tyrosine phosphorylation-dependent activation of TRPC6 regulated by PLC-γ1 and nephrin: effect of mutations associated with focal segmental glomerulosclerosis. |
| 掲載誌名 | 正式名：Molecular biology of the cell 略 称：Mol Biol Cell ISSNコード：19394586/10591524 |
| 掲載区分 | 国外 |
| 巻・号・頁 | 22(11),1824-35頁 |
| 著者・共著者 | Kanda Shoichiro, Harita Yutaka, Shibagaki Yoshio, Sekine Takashi, Igarashi Takashi, Inoue Takafumi, Hattori Seisuke |
| 発行年月 | 2011/06 |
| 概要 | Mutations in the TRPC6 gene are found in patients with focal segmental glomerulosclerosis (FSGS), a proteinuric disease characterized by dysregulated function of renal glomerular epithelial cells (podocytes). We demonstrate here that tyrosine phosphorylation of TRPC6 induces a complex formation with phospholipase C (PLC)-γ1, which is prerequisite for TRPC6 surface expression. Furthermore, nephrin, an adhesion protein between the foot processes of podocytes, binds to phosphorylated TRPC6 via its cytoplasmic domain, competitively inhibiting TRPC6-PLC-γ1 complex formation, TRPC6 surface localization, and TRPC6 activation. Importantly, FSGS-associated mutations render the mutated TRPC6s insensitive to nephrin suppression, thereby promoting their surface expression and channel activation. |
| DOI | 10.1091/mbc.E10-12-0929 |
| PMID | 21471003 |