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カワムラ ヒデマサ
KAWAMURA Hidemasa
河村 英将 所属 埼玉医科大学 医学部 総合医療センター 放射線科(画像診断・核医学科、放射線腫瘍科) 職種 教授 |
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| 論文種別 | 学術雑誌(原著) |
| 言語種別 | 英語 |
| 査読の有無 | 査読なし |
| 表題 | Double-layer omics analysis of castration- and X-ray-resistant prostate cancer cells. |
| 掲載誌名 | 正式名：Journal of radiation research |
| 掲載区分 | 国外 |
| 巻・号・頁 | 63(4),585-590頁 |
| 著者・共著者 | Mototaro Iwanaga,Hidemasa Kawamura,Nobuteru Kubo,Tatsuji Mizukami,Takahiro Oike,Hiro Sato,Yoshiyuki Miyazawa,Yoshitaka Sekine,Reika Kawabata-Iwakawa,Masahiko Nishiyama,Tatsuya Ohno,Takashi Nakano |
| 発行年月 | 2022/05/20 |
| 概要 | Castration-resistant prostate cancer shows resistance to not only androgen deprivation therapy (ADT) but also X-ray therapy. On the other hand, carbon ion beams have a high biological effect and are used for various cancers showing resistance to X-ray therapy. The purposes of this study are to clarify the difference in the sensitivity of Castration-resistant prostate cancer to X-ray and carbon ion beams and to elucidate the mechanism. The androgen-insensitive prostate cancer cell line LNCaP-LA established by culturing the androgen-sensitive prostate cancer cell line LNCaP for 2 years in androgen-free medium was used for this study. First, colony formation assays were performed to investigate its sensitivity to X-ray and carbon ion beams. Next, DNA mutation analysis on 409 cancer-related genes and comprehensive transcriptome analysis (RNA-seq) were performed with a next-generation sequencer. Lethal dose 50 values of X-rays for LNCaP and LNCaP-LA were 1.4 Gy and 2.8 Gy, respectively (P < 0.01). The Lethal dose 50 values of carbon ion beams were 0.9 Gy and 0.7 Gy, respectively (P = 0.09). On DNA mutation analysis, AR mutation was observed specifically in LNCaP-LA. From RNA-seq, 181 genes were identified as differentially expressed genes (DEGs; FDR <0.10, P < 0.00076) between LNCaP and LNCaP-LA. Function analysis suggested that cell death was suppressed in LNCaP-LA, and pathway analysis suggested that the NRF2-pathway involved in intracellular oxidative stress prevention was activated in LNCaP-LA. LNCaP-LA showed X-ray resistance compared to LNCaP and sensitivity to carbon ion beams. The AR mutation and the NRF2-pathway were suggested as causes of resistance. |
| DOI | 10.1093/jrr/rrac022 |
| PMID | 35589101 |