タキザワ　マキコ   TAKIZAWA Makiko   滝沢　牧子    所属   埼玉医科大学  医学部 総合医療センター　医療安全管理学    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Regulation of AID expression in the immune response.
掲載誌名	正式名：The Journal of experimental medicine ISSNコード：0022-1007
掲載区分	国外
巻・号・頁	204(5),1145-56頁
著者・共著者	Elizabeth E Crouch,Zhiyu Li,Makiko Takizawa,Stefan Fichtner-Feigl,Polyxeni Gourzi,Carolina Montaño,Lionel Feigenbaum,Patrick Wilson,Siegfried Janz,F Nina Papavasiliou,Rafael Casellas
発行年月	2007/05/14
概要	The B cell-specific enzyme activation-induced cytidine deaminase (AID) has been shown to be essential for isotype switching and affinity maturation of antibody genes during the immune response. Conversely, AID activity has also been linked to autoimmunity and tumorigenesis. Determining how AID expression is regulated in vivo is therefore central to understanding its role in health and disease. Here we use phylogenetic footprinting and high-resolution histone acetylation mapping to accurately demarcate AID gene regulatory boundaries. Based on this strategy, we identify a novel, positive regulatory element required for AID transcription. Furthermore, we generate two AID indicator mouse strains using bacterial artificial chromosomes that faithfully recapitulate endogenous AID expression. The first strain uses a green fluorescent protein reporter to identify B cells that actively express AID during the immune response. In the second strain, AID transcription affects the permanent expression of a yellow fluorescent protein reporter in post-germinal center and terminally differentiated lymphocytes. We demonstrate the usefulness of these novel strains by resolving recent contradictory observations on AID expression during B cell ontogeny.
PMID	17452520