タキザワ　マキコ   TAKIZAWA Makiko   滝沢　牧子    所属   埼玉医科大学  医学部 総合医療センター　医療安全管理学    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Genetic reporter system for oncogenic Igh-Myc translocations in mice
掲載誌名	正式名：Oncogene ISSNコード：0950-9232
出版社	NATURE PUBLISHING GROUP
巻・号・頁	29(28),4113-4120頁
著者・共著者	M. Takizawa,J. S. Kim,L. Tessarollo,N. McNeil,T. J. Waldschmidt,R. Casellas,T. Ried,S. Janz
発行年月	2010/07
概要	The Myc-deregulating chromosomal T(12; 15)(Igh-Myc) translocation, the hallmark mutation of inflammation-and interleukin 6-dependent mouse plasmacytoma (PCT), is the premier model of cancer-associated chromosomal translocations because it is the only translocation in mice that occurs spontaneously (B lymphocyte lineage) and with predictably high incidence (similar to 85% of PCT), and has a direct counterpart in humans: Burkitt lymphoma t(8;14)(q24;q32) translocation. Here, we report on the development of a genetic system for the detection of T(12;15)(Igh-Myc) translocations in plasma cells of a mouse strain in which an enhanced green fluorescent protein (GFP)-encoding reporter gene has been targeted to Myc. Four of the PCTs that developed in the newly generated translocation reporter mice, designated iGFP(5/Myc), expressed GFP consequent to naturally occurring T(12;15) translocation. GFP expression did not interfere with tumor development or the deregulation of Myc on derivative 12 of translocation, der (12), because the reporter gene was allocated to the reciprocal product of translocation, der (15). Although the described reporter gene approach requires refinement before T(12;15) translocations can be quantitatively detected in vivo, including in B lymphocyte lineage cells that have not yet completed malignant transformation, our findings provide proof of principle that reporter gene tagging of oncogenes in gene-targeted mice can be used to elucidate unresolved questions on the occurrence, distribution and trafficking of cells that have acquired cancer-causing chromosomal translocations of great relevance for humans. Oncogene (2010) 29, 4113-4120; doi:10.1038/onc.2010.150; published online 10 May 2010
DOI	10.1038/onc.2010.150