ヨゴサワ　シンゴ   YOGOSAWA Shingo   与五沢　真吾    所属   埼玉医科大学  保健医療学部 臨床検査学科    職種   准教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Molecular cloning of a novel 120-kDa TBP-interacting protein
掲載誌名	正式名：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS ISSNコード：0006-291X
出版社	ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS
巻・号・頁	229(2),612-617頁
著者・共著者	S Yogosawa,Y Makino,T Yoshida,T Kishimoto,M Muramatsu,T Tamura
発行年月	1996/12
概要	TATA-binding protein (TBP) is a central component for transcriptional regulation and is a target for various transcription regulators. Using histidine-tagged TBP as a ligand for affinity-purification of proteins bound to TBP, we purified a 120-kD protein, termed TBP-interacting protein 120 (TIP120), from rat liver nuclear extracts. The entire cDNA sequence of TIP120 contained an open reading frame encoding a novel polypeptide of 1230 amino acids. The recombinant TIP120 interacted directly with TBP under a physiological condition in vitro. Immunoprecipitation analysis indicated that TIP120 was associated with TBP in nuclear extracts. Interestingly, the N-terminal region of TIP120 exhibited sequence similarity to that of Drosophila TAF80, which was shown to bind directly to TBP. This novel TBP-binding protein is considered to participate in transcription regulation through the interaction with TBP. (C) 1996 Academic Press, Inc.
DOI	10.1006/bbrc.1996.1852
NAID	80009373844
PMID	8954946