ヨゴサワ　シンゴ   YOGOSAWA Shingo   与五沢　真吾    所属   埼玉医科大学  保健医療学部 臨床検査学科    職種   准教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Quercetin induces gadd45 expression through a p53-independent pathway
掲載誌名	正式名：ONCOLOGY REPORTS ISSNコード：1021-335X
出版社	PROFESSOR D A SPANDIDOS
巻・号・頁	14(5),1299-1303頁
著者・共著者	T Yoshida,A Maeda,M Horinaka,T Shiraishi,S Nakata,M Wakada,S Yogosawa,T Sakai
発行年月	2005/11
概要	Quercetin, a kind of flavonoid, is found in edible fruits and vegetables and has anti-tumorigenic activity. However, the mechanism of activity has not been elucidated. We show for the first time that gadd45 is a molecular target of quercetin, which inhibits growth of human cervical cancer HeLa cells. Apoptosis was detected in HeLa cells treated with quercetin. At the concentration inducing apoptosis, quercetin also increased gadd45 expression at the mRNA and protein level, however, the 5'-promoter region of the gadd45 gene was not activated by quercetin. Since gadd45 is known to be a downstream gene of the tumor suppressor p53, we examined whether or not quercetin regulates gadd45 induction via a p53 pathway. Quercetin did not activate transcription through p53-binding sites in HeLa cells, although it up-regulated gadd45 in p53-inactivated tumor cells. These results indicate that quercetin induces gadd45 expression in a p53-independent manner.
PMID	16211300