イマイ　カズオ   IMAI Kazuo   今井　一男    所属   埼玉医科大学  医学部 臨床検査医学（中央検査部）    職種   専任講師
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	A novel diagnostic method for malaria using loop-mediated isothermal amplification (LAMP) and MinION (TM) nanopore sequencer
掲載誌名	正式名：BMC INFECTIOUS DISEASES ISSNコード：14712334
出版社	BIOMED CENTRAL LTD
巻・号・頁	17(1),621頁
著者・共著者	Kazuo Imai,Norihito Tarumoto,Kazuhisa Misawa,Lucky Ronald Runtuwene,Jun Sakai,Kyoko Hayashida,Yuki Eshita,Ryuichiro Maeda,Josef Tuda,Takashi Murakami,Shigefumi Maesaki,Yutaka Suzuki,Junya Yamagishi,Takuya Maeda
発行年月	2017/09
概要	Background: A simple and accurate molecular diagnostic method for malaria is urgently needed due to the limitations of conventional microscopic examination. In this study, we demonstrate a new diagnostic procedure for human malaria using loop mediated isothermal amplification (LAMP) and the MinION (TM) nanopore sequencer.Methods: We generated specific LAMP primers targeting the 18S-rRNA gene of all five human Plasmodium species including two P. ovale subspecies (P. falciparum, P. vivax, P. ovale wallikeri, P. ovale curtisi, P. knowlesi and P. malariae) and examined human blood samples collected from 63 malaria patients in Indonesia. Additionally, we performed amplicon sequencing of our LAMP products using MinION (TM) nanopore sequencer to identify each Plasmodium species.Results: Our LAMP method allowed amplification of all targeted 18S-rRNA genes of the reference plasmids with detection limits of 10-100 copies per reaction. Among the 63 clinical samples, 54 and 55 samples were positive by nested PCR and our LAMP method, respectively. Identification of the Plasmodium species by LAMP amplicon sequencing analysis using the MinION (TM) was consistent with the reference plasmid seque
DOI	10.1186/s12879-017-2718-9
PMID	28903726