カイダ　ケンイチ   KAIDA Kenichi   海田　賢一    所属   埼玉医科大学  医学部 総合医療センター　脳神経内科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Neuropathophysiological potential of Guillain-Barré syndrome anti-ganglioside-complex antibodies at mouse motor nerve terminals
掲載誌名	正式名：Clinical and Experimental Neuroimmunology ISSNコード：17591961
巻・号・頁	2(3),59-67頁
著者・共著者	Femke M. P. Zitman,Kay N. Greenshields,Mark L. Kuijf,Masami Ueda,Ken-Ichi Kaida,Ludo A. M. Broos,Anne P. Tio-Gillen,Bart C. Jacobs,Susumu Kusunoki,Hugh J. Willison,Jaap J. Plomp
発行年月	2011/09
概要	Objectives: Anti-ganglioside antibodies are present in approximately half of Guillain-Barré syndrome (GBS) patients. Recently, it has been shown that a considerable proportion of these patients has serum antibodies against antigenic epitopes formed by a complex of two different gangliosides. However, direct experimental evidence for neuropathogenicity of this special category of antibodies is currently lacking. Here, we explored a series of GBS and GBS-variant sera with anti-ganglioside-complex antibodies for their ability to induce complement-dependent deleterious effects at the living neuronal membrane. Methods: The neuropathophysiological potential of 31 GBS sera containing either anti-GM1/GD1a- or anti-GM1/GQ1b-ganglioside-complex antibodies was studied at motor nerve terminal presynaptic membranes in the mouse phrenic nerve/diaphragm muscle ex vivo experimental model. With electrophysiological measurements and confocal fluorescence microscopy, we assessed and quantified the damaging effect on neuronal membranes by anti-ganglioside-complex antibodies. Results: We show that anti-GM1/GD1a- and anti-GM1/GQ1b-ganglioside-complex positive sera can induce complement-mediated functio
DOI	10.1111/j.1759-1961.2011.00022.x