イチキ　ヨシノブ   ICHIKI Yoshinobu   市来　嘉伸    所属   埼玉医科大学  医学部 国際医療センター　呼吸器外科    職種   専任講師
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読なし
表題	Identification of ribosomal protein L19 as a novel tumor antigenrecognized by autologous cytotoxic T lymphocytes in lung adenocarcinoma.
掲載誌名	正式名：Cancer science
巻・号・頁	101(1),46-53頁
著者・共著者	Koji Kuroda,Mitsuhiro Takenoyama,Tetsuro Baba,Yoshiki Shigematsu,Hironobu Shiota,Yoshinobu Ichiki,Manabu Yasuda,Hidetaka Uramoto,Takeshi Hanagiri,Kosei Yasumoto
発行年月	2010/01
概要	The purpose of the present study was to identify a novel tumor-specific antigen capable of inducing a specific cellular immune response in lung cancer patients. The co-culture of regional lymph node lymphocytes and the CD80-transfected autologous lung adenocarcinoma cell line H1224L resulted in a successful induction of bulk cytotoxic T lymphocytes (CTL). CTL clone L7/8 was established by the limiting dilution method from these bulk CTLs and lysed H1224L but not autologous Epstein-Barr virus-transformed B cells or K562. The CTL clone also recognized allogeneic lung cancer cell lines in an HLA-A*31012-restricted manner. Using the CTL clone, an antigen-coding gene was identified using the cDNA expression cloning technique, which encodes ribosomal protein L19 (RPL19). Finally, a 9 mer antigenic peptide was identified by means of construction of mini-genes. RPL19 was overexpressed in the lung cancer tissue from patient H1224. All of the normal tissues examined expressed lower levels of RPL19 mRNA than that of the lung cancer tissue. RPL19 was also found to be overexpressed in 12 of 30 (40%) non-small-cell lung cancer tissues by immunohistochemical staining. The expression level of RPL1
DOI	10.1111/j.1349-7006.2009.01351.x
PMID	19799608