ジュウジョウ　ケンタロウ   JUJO Kentaro   重城　健太郎    所属   埼玉医科大学  医学部 総合医療センター　心臓内科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Estradiol promotes neural stem cell differentiation into endothelial lineage and angiogenesis in injured peripheral nerve
掲載誌名	正式名：ANGIOGENESIS ISSNコード：09696970
出版社	SPRINGER
巻・号・頁	16(1),45-58頁
著者・共著者	Haruki Sekiguchi,Masaaki Ii,Kentaro Jujo,Tina Thorne,Aiko Ito,Ekaterina Klyachko,Hiromichi Hamada,John A. Kessler,Yasuhiko Tabata,Masatoshi Kawana,Michio Asahi,Nobuhisa Hagiwara,Douglas W. Losordo
発行年月	2013/01
概要	Neural stem cells (NSCs) differentiate into endothelial cells (ECs) and neuronal cells. Estradiol (E2) is known to exhibit proangiogenic effects on ischemic tissues via EC activation. Therefore, we hypothesized that E2 can promote the therapeutic potential of NSC transplantation for injured nerve repair via the differentiation of NSCs into ECs during neovascularization. NSCs isolated from newborn mouse brains were transplanted into injured sciatic nerves with (NSC/E2 group) or without E2-conjugated gelatin hydrogel (E2 group). The NSC/E2 group exhibited the greatest recovery in motor nerve conduction velocity, voltage amplitude, and exercise tolerance. Histological analyses revealed increased intraneural vascularity and blood perfusion as well as striking NSC recruitment to the neovasculature in the injured nerves in the NSC/E2 group. In vitro, E2 enhanced the NSC migration and proliferation inhibiting apoptosis. Fluorescence-activated cell sorting analysis also revealed that E2 significantly increased the percentage of CD31 in NSCs, and the effect of E2 was completely neutralized by the estrogen receptor antagonist ICI. The combination of E2 administration and NSC transplantation
DOI	10.1007/s10456-012-9298-5