セキ　マサフミ   SEK Masafumi   関　雅文    所属   埼玉医科大学  医学部 国際医療センター　感染症科・感染制御科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Multiplex real-time polymerase chain reaction for rapid detection of beta-lactamase-negative, ampicillin-resistant Haemophilus influenzae.
掲載誌名	正式名：Diagnostic microbiology and infectious disease ISSNコード：07328893
巻・号・頁	64(1),64-9頁
著者・共著者	Shigeki Nakamura,Katsunori Yanagihara,Yoshitomo Morinaga,Koichi Izumikawa,Masafumi Seki,Hiroshi Kakeya,Yoshihiro Yamamoto,Shimeru Kamihira,Shigeru Kohno
発行年月	2009/05
概要	We evaluated a multiplex real-time quantitative polymerase chain reaction (PCR) method for quantification of Haemophilus influenzae and rapid detection of beta-lactam-resistant strains. We designed 5 PCR primer sets to simultaneously detect the beta-lactam-resistant genes and quantify the pathogen. To demonstrate the validity of this assay, we used 191 clinical isolates, including 141 H. influenzae strains, and 100 purulent sputum samples, including 30 samples from which H. influenzae had been isolated. This assay showed 92.9% sensitivity and 91.8% specificity for detecting beta-lactam-resistant genes, relative to the conventional phenotypic method, and this assay correlated well with conventional quantitative culture counts. By using this assay, we could quantify H. influenzae and identify beta-lactam susceptibility in only 3 h and with only one tube. This method will be helpful for the rapid detection of H. influenzae infections and the selection of appropriate antibiotics.
DOI	10.1016/j.diagmicrobio.2009.01.023
PMID	19232868