セキ　マサフミ   SEK Masafumi   関　雅文    所属   埼玉医科大学  医学部 国際医療センター　感染症科・感染制御科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読なし
表題	Evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen DNA detection kit
掲載誌名	正式名：CRITICAL CARE ISSNコード：1466609X
出版社	BIOMED CENTRAL LTD
巻・号・頁	14(4),R159頁
著者・共著者	Katsunori Yanagihara,Yuko Kitagawa,Masao Tomonaga,Kunihiro Tsukasaki,Shigeru Kohno,Masafumi Seki,Hisashi Sugimoto,Takeshi Shimazu,Osamu Tasaki,Asako Matsushima,Yasuo Ikeda,Shinichiro Okamoto,Naoki Aikawa,Shingo Hori,Hideaki Obara,Akitoshi Ishizaka,Naoki Hasegawa,Junzo Takeda,Shimeru Kamihira,Kazuyuki Sugahara,Seishi Asari,Mitsuru Murata,Yoshio Kobayashi,Hiroyuki Ginba,Yoshinobu Sumiyama,Masaki Kitajima
発行年月	2010
概要	Introduction: Sepsis is a serious medical condition that requires rapidly administered, appropriate antibiotic treatment. Conventional methods take three or more days for final pathogen identification and antimicrobial susceptibility testing. We organized a prospective observational multicenter study in three study sites to evaluate the diagnostic accuracy and potential clinical utility of the SeptiFast system, a multiplex pathogen detection system used in the clinical setting to support early diagnosis of bloodstream infections.Methods: A total of 212 patients, suspected of having systemic inflammatory response syndrome (SIRS) caused by bacterial or fungal infection, were enrolled in the study. From these patients, 407 blood samples were taken and blood culture analysis was performed to identify pathogens. Whole blood was also collected for DNA Detection Kit analysis immediately after its collection for blood culture. The results of the DNA Detection Kit, blood culture and other culture tests were compared. The chosen antimicrobial treatment in patients whose samples tested positive in the DNA Detection Kit and/or blood culture analysis was examined to evaluate the effect of conco
DOI	10.1186/cc9234
PMID	20731880