セキ　マサフミ   SEK Masafumi   関　雅文    所属   埼玉医科大学  医学部 国際医療センター　感染症科・感染制御科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	High-dose tobramycin inhibits lipopolysaccharide-induced MUC5AC production in human lung epithelial cells.
掲載誌名	正式名：European journal of pharmacology ISSNコード：00142999
巻・号・頁	659(1),67-71頁
著者・共著者	Shigeki Nakamura,Katsunori Yanagihara,Nobuko Araki,Koichi Yamada,Yoshitomo Morinaga,Koichi Izumikawa,Masafumi Seki,Hiroshi Kakeya,Yoshihiro Yamamoto,Shimeru Kamihira,Shigeru Kohno
発行年月	2011/05
概要	Tobramycin inhalation therapy is an effective therapy for cystic fibrosis as well as severe bronchiectasis that is colonized with Pseudomonas aeruginosa. The mechanism responsible for the efficacy of tobramycin in the treatment of severe chronic infectious diseases has not been elucidated. We demonstrate that high-dose tobramycin decreases MUC5AC gene expression and protein production in NCI-H292 cell stimulated with lipopolysaccharide of P. aeruginosa. MUC5AC protein of NCI-H292 cell stimulated by lipopolysaccharide was analyzed by an enzyme-linked immunosorbent assay and MUC5AC expression at the mRNA level was analyzed by RT-PCR. Western blot was performed to examine a potential role for the signaling molecules upstream of NFκB. High-dose tobramycin (500μg/ml) decreased the level of MUC5AC protein released into the supernatant of the NCI-H292 cell line at 24h after lipopolysaccharide stimulation (P<0.001). The tobramycin treatment also inhibited MUC5AC mRNA expression at 12h after lipopolysaccharide stimulation (P<0.05) and suppressed NFκB activation 60min after lipopolysaccharide stimulation (P<0.001). Tobramycin suppressed the phosphorylation of extracellular signal-regulate
DOI	10.1016/j.ejphar.2011.03.002
NAID	120003220709
PMID	21414310