セキ　マサフミ   SEK Masafumi   関　雅文    所属   埼玉医科大学  医学部 国際医療センター　感染症科・感染制御科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Identification of neutrophil extracellular traps in the blood of patients with systemic inflammatory response syndrome.
掲載誌名	正式名：The Journal of international medical research ISSNコード：03000605
出版社	SAGE PUBLICATIONS LTD
巻・号・頁	41(1),162-8頁
著者・共著者	Shigeto Hamaguchi,Tomoya Hirose,Yukihiro Akeda,Naoya Matsumoto,Taro Irisawa,Masafumi Seki,Hideo Hosotsubo,Osamu Tasaki,Kazunori Oishi,Takeshi Shimazu,Kazunori Tomono
発行年月	2013/02
概要	OBJECTIVE: Neutrophils are able to form 'neutrophil extracellular traps' (NETs), which they use to trap and kill pathogens such as bacteria and fungi at the foci of infection. This observational study investigated the presence of NETs in the blood from critically ill patients and healthy volunteers. METHODS: Fluorescent triple-colour immunocytochemical analysis of blood smears collected from patients with systemic inflammatory response syndrome (SIRS; associated with various clinical conditions) who had been hospitalized in the intensive care unit, and healthy volunteers, was undertaken to identify NETs in the blood. Blood smears were stained for DNA, histone H1 and neutrophil elastase. RESULTS: NETs were identified in 10 of 21 (47.6%) blood samples from the study group compared with none of the blood samples from eight healthy volunteers. CONCLUSION: These data suggest that fluorescent triple-colour immunocytochemical staining of NETs in the blood could be used to simplify the early identification of critically ill patients with SIRS. Larger studies are required to clarify the pathophysiological role of NETs in this specific patient population.
DOI	10.1177/0300060513475958
PMID	23569142