セキ　マサフミ   SEK Masafumi   関　雅文    所属   埼玉医科大学  医学部 国際医療センター　感染症科・感染制御科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Presence of neutrophil extracellular traps and citrullinated histone H3 in the bloodstream of critically ill patients.
掲載誌名	正式名：PloS one ISSNコード：19326203
出版社	PUBLIC LIBRARY SCIENCE
巻・号・頁	9(11),e111755頁
著者・共著者	Tomoya Hirose,Shigeto Hamaguchi,Naoya Matsumoto,Taro Irisawa,Masafumi Seki,Osamu Tasaki,Hideo Hosotsubo,Norihisa Yamamoto,Kouji Yamamoto,Yukihiro Akeda,Kazunori Oishi,Kazunori Tomono,Takeshi Shimazu
発行年月	2014
概要	Neutrophil extracellular traps (NETs), a newly identified immune mechanism, are induced by inflammatory stimuli. Modification by citrullination of histone H3 is thought to be involved in the in vitro formation of NETs. The purposes of this study were to evaluate whether NETs and citrullinated histone H3 (Cit-H3) are present in the bloodstream of critically ill patients and to identify correlations with clinical and biological parameters. Blood samples were collected from intubated patients at the time of ICU admission from April to June 2011. To identify NETs, DNA and histone H3 were visualized simultaneously by immunofluorescence in blood smears. Cit-H3 was detected using a specific antibody. We assessed relationships of the presence of NETs and Cit-H3 with the existence of bacteria in tracheal aspirate, SIRS, diagnosis, WBC count, and concentrations of IL-8, TNF-α, cf-DNA, lactate, and HMGB1. Forty-nine patients were included. The median of age was 66.0 (IQR: 52.5-76.0) years. The diagnoses included trauma (7, 14.3%), infection (14, 28.6%), resuscitation from cardiopulmonary arrest (8, 16.3%), acute poisoning (4, 8.1%), heart disease (4, 8.1%), brain stroke (8, 16.3%), heat stro
DOI	10.1371/journal.pone.0111755
PMID	25392950