セキ　マサフミ   SEK Masafumi   関　雅文    所属   埼玉医科大学  医学部 国際医療センター　感染症科・感染制御科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Neutrophil extracellular traps in bronchial aspirates: a quantitative analysis.
掲載誌名	正式名：The European respiratory journal ISSNコード：09031936
出版社	EUROPEAN RESPIRATORY SOC JOURNALS LTD
巻・号・頁	43(6),1709-18頁
著者・共著者	Shigeto Hamaguchi,Tomoya Hirose,Naoya Matsumoto,Yukihiro Akeda,Taro Irisawa,Masafumi Seki,Hideo Hosotsubo,Kouji Yamamoto,Osamu Tasaki,Kazunori Oishi,Takeshi Shimazu,Kazunori Tomono
発行年月	2014/06
概要	Neutrophil extracellular traps (NETs) are structures composed of DNA and granular proteins, which rapidly trap and kill pathogens. The formation of NETs has been detected during infection in animal experiments, but their role in humans is unclear. The purposes of this study were to quantitatively evaluate the production of NETs during acute respiratory infection and to study the relationship between the NET length and various inflammatory mediators. We examined bronchial aspirates collected from nine intubated patients in an intensive care unit. Samples were collected at the onset of acute respiratory infection (day 0) and on days 1, 3-5, and 6-8. The NET length was visualised by immunohistochemistry and quantified using computer tracing software. The NET length was measured and compared at each time point. The length differed significantly between time points (p<0.001). NETs were significantly longer on day 1 than on day 0 (p<0.001). Neutrophils released NETs abundantly in response to respiratory infection and regression analysis showed that NET length correlated with six clinical parameters (white blood cells, platelets, lactate, CXC ligand-2, interleukin-8, and procalcitonin) as
DOI	10.1183/09031936.00139813
PMID	24603817