セキ　マサフミ   SEK Masafumi   関　雅文    所属   埼玉医科大学  医学部 国際医療センター　感染症科・感染制御科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Validation of a phage-open reading frame typing kit for rapid identification of methicillin-resistant Staphylococcus aureus (MRSA) transmission in a tertiary hospital.
掲載誌名	正式名：Infection and drug resistance ISSNコード：11786973
出版社	Dove Medical Press Ltd.
巻・号・頁	8,107-11頁
著者・共著者	Hiroki Takahashi,Masafumi Seki,Norihisa Yamamoto,Shigeto Hamaguchi,Masahiro Ojima,Tomoya Hirose,Kazuhisa Yoshiya,Masahiro Toyokawa,Isao Nishi,Hiroshi Ogura,Takeshi Shimazu,Kazunori Tomono
発行年月	2015
概要	Surveillance is very important to prevent the nosocomial spread of methicillin-resistant Staphylococcus aureus (MRSA), and infection sources and routes have historically been identified using molecular and epidemiological genotyping with pulsed-field gel electrophoresis. However, phage-open reading frame typing (POT) has recently been developed. Here, we investigated whether POT would be useful to survey MRSA outbreaks and transmission. We therefore applied POT to 91 MRSA isolates detected in cultures from inpatients at our hospital between May and October 2014. Among the 91 isolates, 12 POT types comprising 38 isolated MRSA strains were considered as overlapping. Five of them were detected in different wards, whereas the remaining seven were found in the same ward, including the emergency department. Three of seven POT number 93-155-111 strains were detected in the surgical ward, and all of four POT number 93-157-61 strains were detected in the cardiosurgical ward. These data suggested that transmission of the MRSA strains with the same POT-types from the same wards was nosocomial, and that POT accurately and rapidly identified MRSA strains, which allowed effective control of infe
DOI	10.2147/IDR.S83509
PMID	25999746