セキ　マサフミ   SEK Masafumi   関　雅文    所属   埼玉医科大学  医学部 国際医療センター　感染症科・感染制御科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Clinical Specimen-Direct LAMP: A Useful Tool for the Surveillance of bla(OXA-23)-Positive Carbapenem-Resistant Acinetobacter baumannii
掲載誌名	正式名：PLOS ONE ISSNコード：19326203
出版社	PUBLIC LIBRARY SCIENCE
巻・号・頁	10(7),e0133204頁
著者・共著者	Norihisa Yamamoto,Shigeto Hamaguchi,Yukihiro Akeda,Pitak Santanirand,Anusak Kerdsin,Masafumi Seki,Yoshikazu Ishii,Wantana Paveenkittiporn,Robert A. Bonomo,Kazunori Oishi,Kumthorn Malathum,Kazunori Tomono
発行年月	2015/07
概要	Healthcare-associated infections are a leading cause of morbidity and mortality worldwide. Treatment is increasingly complicated by the escalating incidence of antimicrobial resistance. Among drug-resistant pathogens, carbapenem-resistant Acinetobacter baumannii (CRAb) is of increasing concern because of the limited applicable therapies and its expanding global distribution in developed countries and newly industrialized countries. Therefore, a rapid detection method that can be used even in resource-poor countries is urgently required to control this global public health threat. Conventional techniques, such as bacterial culture and polymerase chain reaction (PCR), are insufficient to combat this threat because they are time-consuming and laborious. In this study, we developed a loop-mediated isothermal amplification (LAMP) method for detecting bla(OXA-23)-positive CRAb, the most prevalent form of CRAb in Asia, especially in Thailand, and confirmed its efficacy as a surveillance tool in a clinical setting. Clinical samples of sputum and rectal swabs were collected from patients in a hospital in Bangkok and used for LAMP assays. After boiling and centrifugation, the supernatants we
DOI	10.1371/journal.pone.0133204
PMID	26218925