オノ　コウジ   ONO Koji   小野　公嗣    所属   埼玉医科大学  保健医療学部 臨床検査学科    職種   准教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Nucling, a novel protein associated with NF-kappa B, regulates endotoxin-induced apoptosis in vivo
掲載誌名	正式名：JOURNAL OF BIOCHEMISTRY ISSNコード：0021924X
出版社	OXFORD UNIV PRESS
巻・号・頁	153(1),93-101頁
著者・共著者	Sun Mi Kim,Takashi Sakai,Huy Van Dang,Nam Hoang Tran,Koji Ono,Kazunori Ishimura,Kiyoshi Fukui
発行年月	2013/01
概要	Nucling is a proapoptotic protein that regulates the apoptosome and nuclear factor-kappa B (NF-kappa B) signalling pathways. Strong stimuli, such as Gram-negative bacterial lipopolysaccharide (LPS), induce the simultaneous secretion of cytokines following the activation of NF-kappa B. Proinflammatory cytokines can induce liver damage through several mechanisms such as increases in oxidative stress and apoptotic reactions leading to tissue necrosis. Herein, we show that Nucling-knockout (KO) mice are resistant to LPS that consistently caused mortality in wild-type (WT) counterparts. Although serum levels of cytokines such as tumour necrosis factor (TNF)-alpha, interleukin (IL)-1 beta and IL-6 did not differ significantly between WT and Nucling-KO mice after the LPS challenge, hepatocytes of Nucling-KO mice were refractory to LPS- or TNF-alpha-induced cell death. These results were consistent with the decreased expression of proapoptotic proteins including apoptosis-inducing factor and cleaved form of poly (ADP-ribose) polymerase and terminal deoxynucleotidyl transferase dUTP nick end-labelling positive cells in the liver of Nucling-KO mice after the administration of a lethal dose o
DOI	10.1093/jb/mvs119