オバタ　ヒデアキ   OBATA Hideaki   小幡　英章    所属   埼玉医科大学  医学部 総合医療センター　麻酔科（麻酔科、産科麻酔科）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Intracellular calcium increases in growth cones exposed to tetracaine
掲載誌名	正式名：ANESTHESIA AND ANALGESIA ISSNコード：00032999
出版社	LIPPINCOTT WILLIAMS&WILKINS
巻・号・頁	98(3),841-845頁
著者・共著者	S Saito,IAM Radwan,K Nishikawa,H Obata,T Okamoto,T Kanno,F Goto
発行年月	2004/03
概要	Neurotoxicity of local anesthetics has been reported for both matured and growing neurons. In the resent study, we examined if tetracaine increases Ca2+ concentration during growth cone collapse. Intracellular Ca2+ concentration was measured by fura 2/AM after exposure to tetracaine. Tetracaine (1-2 mM) induced increases in intra-growth cone Ca2+ concentration (P< 0.01). The Ca2+ hot spot was expanded into the neurite from the periphery towards the cell body. When tetracaine was applied to growth cones in Ca2+ free the increase was minor. However, tetracaine induced growth cone collapse even in the culture media, which did not contain Ca2+. Ni2+ (100 muM; a general Ca2+ channel inhibitor) and BAPTA-AM (5 muM; intracellular Ca2+ chelator) could not inhibit growth cone collapse induced by 1-2 mM tetracaine. Tetracaine (>1 mM) induces collapse and Ca2+ increase at growth cones simultaneously; however, these two phenomena might be provoked independently.
PMID	14980949