オバタ　ヒデアキ   OBATA Hideaki   小幡　英章    所属   埼玉医科大学  医学部 総合医療センター　麻酔科（麻酔科、産科麻酔科）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Direct neurotoxicity of tetracaine on growth cones and neurites of growing neurons in vitro
掲載誌名	正式名：ANESTHESIOLOGY ISSNコード：00033022
出版社	LIPPINCOTT WILLIAMS&WILKINS
巻・号・頁	95(3),726-733頁
著者・共著者	S Saito,Radwan, I,H Obata,K Takahashi,F Goto
発行年月	2001/09
概要	Background: Local anesthetics have direct neurotoxicity on neurons. However, precise morphologic changes induced by the direct application of local anesthetics to neurons have not yet been fully understood. Also, despite the fact that local anesthetics are sometimes applied to the sites where peripheral nerves may be regenerating after injury, the effects of local anesthetics on growing or regenerating neurons have never been studied.Methods: Three different neuronal tissues (dorsal root ganglion, retinal ganglion cell layer, and sympathetic ganglion chain) were isolated from an age-matched chick embryo and cultured for 20 h. Effects of tetracaine were examined microscopically and by a quantitative morphologic assay, growth cone collapse assay.Results: Tetracaine induced growth cone collapse and neurite destruction. Three neuronal tissues showed significantly different dose-response, both at 60 min and at 24 h after the application of tetracaine (P< 0.01). The ED50 values (mean +/- SD) at 60 min were 1.53 +/- 1.05 mM in dorsal root ganglion, 0.15 +/- 0.05 mM in retinal, and 0.06 +/- 0.02 mM In sympathetic ganglion chain cultures. The ED50 values at 24 h were 0.43 +/- 0.15 mM
PMID	11575547