コレマツ　セイゴ   KOREMATSU Seigo   是松　聖悟    所属   埼玉医科大学  医学部 総合医療センター　小児科（小児科､総合周産期母子医療センター新生児科、小児救命救急センター）    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Crystal structure and some properties of a major house dust mite allergen, Derf 2.
掲載誌名	正式名：Biochemical and biophysical research communications ISSNコード：0006291X
出版社	ACADEMIC PRESS INC ELSEVIER SCIENCE
巻・号・頁	339(2),679-86頁
著者・共著者	Masashi Suzuki,Yoshimasa Tanaka,Seigo Korematsu,Bunzo Mikami,Nagahiro Minato
発行年月	2006/01
概要	Pyroglyphid house dust mites are a major source of allergens in house dust. Mite allergens sensitize and induce asthma, rhinitis, and eczema in a large portion of patients with allergic diseases. Here, the crystal structure of a major mite allergen, Derf 2, derived from Dermatophagoides farinae was solved by single isomorphous replacement method with anomalous scattering (SIRAS) at 2.1A resolution. The present study also demonstrated that the conformation of the allergen was critical in the determination of Th1/Th2 shift based on physicochemical and immunological analyses. This indicates that rigidly folded and singly dispersed structure is essentially required for the generation of Th2 type cells by the allergen, while conformational variant protein leads to Th1 skewing, irrespective of the same amino acid sequence. This structure/function relationship may allow us to develop a novel strategy for hyposensitization therapy in patients with allergic diseases triggered by house dust mite allergens.
DOI	10.1016/j.bbrc.2005.11.065
NAID	80017698432
PMID	16313885