ミヤマ　ユウ   MIYAMA Yu   美山　優    所属   埼玉医科大学  医学部 国際医療センター　病理診断科    職種   助教
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Fluorescence Detection of Prostate Cancer by an Activatable Fluorescence Probe for PSMA Carboxypeptidase Activity.
掲載誌名	正式名：Journal of the American Chemical Society
巻・号・頁	141(26),10409-10416頁
著者・共著者	Minoru Kawatani,Kyoko Yamamoto,Daisuke Yamada,Mako Kamiya,Jimpei Miyakawa,Yu Miyama,Ryosuke Kojima,Teppei Morikawa,Haruki Kume,Yasuteru Urano
発行年月	2019/07
概要	Prostate cancer (PCa) is a common malignant tumor among adult males, and convenient intraoperative detection of PCa would reduce the risk of leaving positive surgical margins, especially during nerve-sparing procedures. To achieve rapid, fluorescence-based visualization of PCa, we focused on the glutamate carboxypeptidase (CP) activity of prostate-specific membrane antigen (PSMA), a type II transmembrane glycoprotein that is attracting attention as a PCa biomarker. Based on our finding that aryl glutamate conjugates with an azoformyl linker are recognized by PSMA and have a sufficiently low LUMO (lowest unoccupied molecular orbital) energy level to quench the fluorophore through photoinduced electron transfer, we designed and synthesized a first-in-class activatable fluorescence probe for CP activity of PSMA. The developed probe allowed us to visualize the CP activity of PSMA in living cells and in clinical specimens from PCa patients and is expected to be useful for rapid intraoperative detection and diagnosis of PCa.
DOI	10.1021/jacs.9b04412
PMID	31244179