ヤマギシ　トシユキ   YAMAGISHI Toshiyuki   山岸　敏之    所属   埼玉医科大学  保健医療学部 臨床検査学科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Epicardium is required for sarcomeric maturation and cardiomyocyte growth in the ventricular compact layer mediated by transforming growth factor beta and fibroblast growth factor before the onset of coronary circulation
掲載誌名	正式名：CONGENITAL ANOMALIES ISSNコード：09143505
出版社	WILEY
巻・号・頁	54(3),162-171頁
著者・共著者	Makiko Takahashi,Toshiyuki Yamagishi,Mayu Narematsu,Tatsuya Kamimura,Masatake Kai,Yuji Nakajima
発行年月	2014/08
概要	The epicardium, which is derived from the proepicardial organ (PE) as the third epithelial layer of the developing heart, is crucial for ventricular morphogenesis. An epicardial deficiency leads to a thin compact layer for the developing ventricle; however, the mechanisms leading to the impaired development of the compact layer are not well understood. Using chick embryonic hearts, we produced epicardiumdeficient hearts by surgical ablation or blockade of the migration of PE and examined the mechanisms underlying a thin compact myocardium. Sarcomeric maturation (distance between Z-lines) and cardiomyocyte growth (size) were affected in the thin compact myocardium of epicardium-deficient ventricles, in which the amounts of phospho-smad2 and phosphoERK as well as expression of transforming growth factor (TGF)beta 2 and fibroblast growth factor (FGF) 2 were reduced. TGF beta and FGF were required for the maturation of sarcomeres and growth of cardiomyocytes in cultured ventricles. In ovo co-transfection of dominant negative (dN)-Alk5 (dN-TGF beta receptor I) and dN-FGF receptor 1 to ventricles caused a thin compact myocardium. Our results suggest that immature sarcomeres and small car
DOI	10.1111/cga.12048
PMID	24666202