ヤマギシ　トシユキ   YAMAGISHI Toshiyuki   山岸　敏之    所属   埼玉医科大学  保健医療学部 臨床検査学科    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Nodal signal is required for morphogenetic movements of epiblast layer in the pre-streak chick blastoderm
掲載誌名	正式名：DEVELOPMENT GROWTH&DIFFERENTIATION ISSNコード：00121592
出版社	WILEY-BLACKWELL
巻・号・頁	53(3),366-377頁
著者・共著者	Nariaki Yanagawa,Masahide Sakabe,Hirokazu Sakata,Toshiyuki Yamagishi,Yuji Nakajima
発行年月	2011/04
概要	During axis formation in amniotes, posterior and lateral epiblast cells in the area pellucida undergo a counter-rotating movement along the midline to form primitive streak (Polonaise movements). Using chick blastoderms, we investigated the signaling involved in this cellular movement in epithelial-epiblast. In cultured posterior blastoderm explants from stage X to XI embryos, either Lefty1 or Cerberus-S inhibited initial migration of the explants on chamber slides. In vivo analysis showed that inhibition of Nodal signaling by Lefty1 affected the movement of DiI-marked epiblast cells prior to the formation of primitive streak. In Lefty1-treated embryos without a primitive streak, Brachyury expression showed a patchy distribution. However, SU5402 did not affect the movement of DiI-marked epiblast cells. Multi-cellular rosette, which is thought to be involved in epithelial morphogenesis, was found predominantly in the posterior half of the epiblast, and Lefty1 inhibited the formation of rosettes. Three-dimensional reconstruction showed two types of rosette, one with a protruding cell, the other with a ventral hollow. Our results suggest that Nodal signaling may have a pivotal role in
DOI	10.1111/j.1440-169X.2010.01244.x
PMID	21492150