所属 埼玉医科大学 保健医療学部 臨床検査学科 職種 教授
|Tenascin C may regulate the recruitment of smooth muscle cells during coronary artery development
|ELSEVIER SCI LTD
|Katsumi Ando,Makiko Takahashi,Toshiyuki Yamagishi,Sachiko Miyagawa-Tomita,Kyoko Imanaka-Yoshida,Toshimichi Yoshida,Yuji Nakajima
|Tenascin C (TNC) is an extracellular glycoprotein that is thought tobe involved in tissue remodeling during organogenesis and regeneration. Using avian embryonic hearts, we investigated the spatio-temporal expression patterns of TNC during the formation of the proximal coronary artery. Immunohistochemistry showed that TNC was deposited around the developing coronary stem and that TNC colocalized with vascular smooth muscle alpha-actin. Aquail-chick chimera, in which a quail proepicardial organ (PEO) had been transplanted, showed that quail tissue-derived cells contributed to the establishment of the endothelial and mural cells of the proximal coronary artery, and the quail tissue-derived mural cells displayed TNC. Proepicardial cells cultured in TNC showed the myofibro-blast/smooth muscle cell phenotype and neutralizing anti-TNC antibody suppressed the expression of smooth muscle markers. These observations suggest that TNC plays a role in the mural smooth muscle development of the nascent proximal coronary artery. (C) 2011 International Society of Differentiation. Published by Elsevier Ltd. All rights reserved.