カワムラ　ヒデマサ   KAWAMURA Hidemasa   河村　英将    所属   埼玉医科大学  医学部 総合医療センター　放射線科(画像診断・核医学科、放射線腫瘍科)    職種   教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	Ceramide Induces Myogenic Differentiation and Apoptosis in Drosophila Schneider Cells
掲載誌名	正式名：JOURNAL OF RADIATION RESEARCH ISSNコード：0449-3060
出版社	JAPAN RADIATION RESEARCH SOC
巻・号・頁	50(2),161-169頁
著者・共著者	Hidemasa Kawamura,Kazuaki Tatei,Tetsuo Nonaka,Hideru Obinata,Tomoyasu Hattori,Ai Ogawa,Hideko Kazama,Nobuyuki Hamada,Tomoo Funayama,Tetsuya Sakashita,Yasuhiko Kobayashi,Takashi Nakano,Takashi Izumi
発行年月	2009/03
概要	Cells exposed to genotoxic stress, such as ionizing radiation and DNA damaging reagents, either arrest the cell cycle to repair the genome, or undergo apoptosis, depending on the extent of the DNA damage. DNA damage also has been implicated in various differentiation processes. It has been reported that gamma-ray exposure or treatment with DNA-damaging agents could induce myogenic differentiation in Drosophila Schneider cells. However, the mechanism underlying this process has been poorly understood. In this study, exposure of Schneider cells to X-rays or energetic carbon ion beams caused increase of TUNEL-positive cells and conversion of round-shaped cells to elongated cells. Both upregulation of genes related to myogenesis and increase of myosin indicate that the radiation-induced morphological changes of Schneider cells were accompanied with myogenic differentiation. Because the intracellular ceramide was increased in Schneider cells after exposure to X-ray, we examined whether exogenous ceramide could mimic radiation-induced myogenic differentiation. Addition of membrane-permeable C(2)-ceramide to Schneider cells increased apoptosis and expression of myogenic genes. These results suggest that ceramide plays important roles in both apoptosis and the radiation-induced myogenic differentiation process.
DOI	10.1269/jrr.08122
PMID	19346679