マツサカ　ヨウジ   MATSUSAKA Yohji   松坂　陽至    所属   埼玉医科大学  医学部 国際医療センター　放射線科（放射線腫瘍科、画像診断、核医学科）    職種   准教授
論文種別	学術雑誌(原著)
言語種別	英語
査読の有無	査読あり
表題	F-18-FDG-labeled red blood cell PET for blood-pool imaging: preclinical evaluation in rats
掲載誌名	正式名：EJNMMI RESEARCH ISSNコード：2191219X
出版社	SPRINGER HEIDELBERG
巻・号・頁	7(1),19頁
著者・共著者	Yohji Matsusaka,Tadaki Nakahara,Kazuhiro Takahashi,Yu Iwabuchi,Chiyoko Nishime,Mayumi Kajimura,Masahiro Jinzaki
発行年月	2017/02
概要	Background: Red blood cells (RBCs) labeled with single-photon emitters have been clinically used for blood-pool imaging. Although some PET tracers have been introduced for blood-pool imaging, they have not yet been widely used. The present study investigated the feasibility of labeling RBCs with F-18-2-deoxy-2-fluoro-D-glucose(F-18-FDG) for blood-pool imaging with PET.RBCs isolated from venous blood of rats were washed with glucose-free phosphate-buffered saline and labeled with F-18-FDG. To optimizelabeling efficiency, the effects of glucose deprivation time and incubation (labeling) time with F-18-FDG were investigated. Post-labeling stability was assessed by calculating the release fraction of radioactivity and identifying the chemical forms of F-18 in the released and intracellular components of F-18-FDG-labeled RBCs incubated in plasma. Just after intravenous injection of the optimized autologous F-18-FDG-labeled RBCs, dynamic PET scans were performed to evaluate in vivo imaging in normal rats and intraabdominal bleeding models (temporary and persistent bleeding).Results: The optimal durations of glucose deprivation and incubation (labeling) with F-18-FDG were 60 and 30 min,
DOI	10.1186/s13550-017-0266-3
PMID	28244021