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マツサカ ヨウジ
MATSUSAKA Yohji
松坂 陽至 所属 埼玉医科大学 医学部 国際医療センター 放射線科（放射線腫瘍科、画像診断、核医学科） 職種 准教授 |
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| 論文種別 | 学術雑誌(原著) |
| 言語種別 | 英語 |
| 査読の有無 | 査読あり |
| 表題 | Preclinical evaluation of heat-denatured [18F]FDG-labeled red blood cells for detecting splenic tissues with PET in rats |
| 掲載誌名 | 正式名：Nuclear Medicine and Biology ISSNコード：18729614 |
| 出版社 | Elsevier Inc. |
| 巻・号・頁 | 56,26-30頁 |
| 著者・共著者 | Yohji Matsusaka,Tadaki Nakahara,Kazuhiro Takahashi,Yu Iwabuchi,Yuji Ogata,Chiyoko Nishime,Mayumi Kajimura,Masahiro Jinzaki |
| 発行年月 | 2018/01 |
| 概要 | Introduction Heat-denatured 99mTc-labeled red blood cells (RBCs) are used for detecting splenic tissues with scintigraphy. The present study aimed to evaluate the feasibility of using heat-denatured [18F]fluorodeoxyglucose ([18F]FDG)-labeled RBCs in detecting splenic tissues using positron emission tomography (PET) in rats. Methods RBCs were washed with phosphate buffered saline, labeled with [18F]FDG at 38°C, and heat-denatured at 50°C for 15 min. In vitro stability was assessed by measuring extracellular radioactivity during the 0–180 min incubation at 37°C. Thin layer chromatography (TLC) of the extracellular fluid was performed. The autologous RBCs were intravenously injected in four rats and PET scanning was simultaneously performed for 30 min. Time-activity curves of several organs, including the spleen, were analyzed on the PET images. Results Labeling efficiency was 92%. Low levels of radioactivity were released from the labeled RBCs for 180 min. TLC revealed that 80% of the released radioactivity was due to [18F]FDG-6-phosphate. Whole body images showed strong uptake of heat-denatured [18F]FDG-labeled RBCs in the spleen soon after injection in all four rats. Time-acti |
| DOI | 10.1016/j.nucmedbio.2017.09.002 |
| PMID | 29125999 |